Thursday, January 30, 2020
On the Sidewalk Bleeding Monuloge Essay Example for Free
On the Sidewalk Bleeding Monuloge Essay All I feel is the excruciating pain entering my aching body. The irritation and agony makes me feel defenceless. I have a multitude of varied thoughts racing through my confused mind. As I am helplessly laying on the cold, wet concrete, with the rain drilling down on me, I am thinking if this is how I am going to die, my life over at the age of just 16. I can think back and faintly remember someone saying, ââ¬Å"thatââ¬â¢s for you royal!â⬠and just thinking to myself, if I was not wearing this stupid jacket that I once thought was so important, I perchance wouldnââ¬â¢t have even been in this discomforting situation. All I can do at this point is helplessly lay here praying for someone to find me. Then finally I take a great effort and look over to the end of the street though the rain blurring my vision and see the bright neon lights of a vehicle binding me. I feel a massive relief, I see two figures coming toward me I try to yell again but they seem to just be talking to each other. I want to get their attention but all the sound I can make is the bubbling of blood filing my mouth, as if I am drowning in my own vital fluid. It sounds like the grunt of an animal and thatââ¬â¢s the only sound I am capable of making in this state. I start to panic because the people arenââ¬â¢t recognising that I am there, after I take all my energy and frustration to get their attention they look at me for a little then they mutter to each other, I canââ¬â¢t understand much they are mumbling about. I just feel powerless hoping he will make the right decision and just get a cop or hep me. He looks at me, my cut body and the rain soaking my purple royals jacket. He sympathetically says, ââ¬Å"Sorry royal,â⬠and walks away. At this very moment I feel frustrated, overwhelmingly confused with anger and abundance. Is it that big of a deal that I am a royal? I am not just a royal, I am Andy. I feel drastically judged and labelled. Right now I will do anything and everything I am physically capable of just to take this jacket off, the only thing that jacket ever did to me was rob me of my life.
Wednesday, January 22, 2020
The Art of Leadership Essay -- Papers Leading Personality Essays Paper
The Art of Leadership What is leadership? Leadership is having the ability to give guidance to those that will follow. Those that follow will help to complete the mission. Leadership is an immature science and the body of knowledge in the field has developed through a series of fits and starts. Leadership is a soft science, just as anthropology, sociology and psychology. It cannot be proven exactly what it is. Leadership is an art; the skillful application of leadership behaviors beyond techniques is much the same as the skillful application of brushstrokes by a master painter. Leadership is both rational and emotional. Some people take several years and even several classes and still canââ¬â¢t quite be a good leader. I even took a test, which more or less states that I need to relax and calm down. The test covers several different areas ranging from personality quirks to communication and even how I prefer to handle conflicts. Introduction This paper will be discussing several areas that were examined through the test on leadership and personality that was taken by the author. Some of the major points that will be discussed are how the author rates himself against others on his team or in his group. A couple of other major points that will be touched on are conflict management and communication skills. Also, the author will be covering some of his ideas on leadership and whether or not any one individual can perfect it. The author has quite a bit of experience in these areas and conveyed some of his ideas in this paper. Strategy Leadership is a social process shared among all members of a group. Leadership development comes t... ...e people to totally change their way of doing things but if there are some problems, I hope those individuals were able to pull some ideas out of this in the long run. Conclusion While writing this paper I concluded several different things about leadership. Most people are very mistaken when it comes to the topic of leadership. It is a common belief that everyone has the potential to be a good leader, which is not true. This is proven time and time again by people who try to start their own business and it goes under in one or two years because they donââ¬â¢t know how to get the most out of their employees and keep them happy at the same time. In the end, a leader will always accomplish the mission and never fail his subordinates. Bibliography: 1. Robbins, Stephen P. Organizational Behavior. Upper Saddle River, NJ: Prentice. Hall, 2000.
Tuesday, January 14, 2020
How Social Networking Has Affected the Way We Work Essay
Social networks are a fun way to communicate, interact with friends, and enjoy online schooling, but are also harmful due to the harassment of cyber bullies and predators. Social networks/medias show their good side when we are all having fun on them, communicating with our friends. It is a fun and interactive way to converse, and meet new people; it gives people opportunities to learn characteristics that they have in common with each other, and make stronger relationships. Some people use Facebook, Twitter, and even Myspace to communicate with their friends; usually it is to make them aware of what youââ¬â¢re doing, going to do, or already have done, and meet new people, all through a fun, inventive way. Cyber Predators find their victims through internet sources, which is often social media, to learn personal information, harass, and stalk their victims; in most cases causing physical, emotional, and/or mental harm to the victim. Cyber School is an effective way to receive an education over the internet and helps to balance a home, school, and work life; getting an education that is flexible to any schedule is a positive development. Social media is a fun and interactive way to converse with friends and meet new people; it gives people opportunities to learn about things that they have in common; and make a stronger relationship. Cyber Bullies and Predators usually affect the lives of teenagers and children because of their vulnerability; ââ¬Å"75% of children are willing to share personal information online about themselves and their families in exchange for goods and services;â⬠A Cyber Bully is someone who uses the internet to torment another person using cruel words and hateful sayings to purposefully discourage them; a Cyber Predator is usually an adult internet abuser that exploits children or teenagers because of their lack of wisdom and their large amount of vulnerability. Others might think that social networking isnââ¬â¢t helpful at all, that there is no use of it, and that it needs to be used less because of the lack of exercise children receive and connection it has to the increasing obesity growth rate or that it has only negative effects on its userââ¬â¢s safety. Social networking has changed how people live, work, and communicate by offering helpful schooling online, fun communication between others, but it also gives bullies and predators another method of victimizing others. Social media, though it may be a fun way to interact with others, can be harmful when it comes to your safety; many people donââ¬â¢t realize that putting much of their information on the internet can invite unwanted problems to your life.Andrew Ferguson, author of the web article, ââ¬Å"R U Tweeting 2 Much?â⬠published by Gale Opposing View Points In Context, in 2011, implies, people spend too much of their time on twitter and they tweet about any and everything based on impulse. Social media can be a powerful tool for social change and an alternative to more traditional methods of communication. ââ¬Å"Consequences from over-sharing personal information include vulnerability to sexual or financial predators and lost job opportunities from employers finding embarrassing photos or comments.â⬠No one would want their boss to see a picture on Facebook of their new employee getting drunk, so why would they put it up for the entire world to see? There is a saying that goes ââ¬Å"If you wouldnââ¬â¢t want your pastor at church, your parents, or your boss to know/see, then donââ¬â¢t do it.â⬠Socialnetworking.procon.org, updated in 2012, clarifies that, ââ¬Å"43% of online sexual solicitors were identified as being adolescents (under 18), 30% were adults between the ages of 18 and 21, and 9% were adults over the age of 21 (as of Dec. 31, 2008).â⬠Predators can be any age; so if someone gets a friend request from a random person that theyââ¬â¢ve never met before, but is the same age as them, they will probably accept the request; they think that because we are the same age, that the person must want to get to know me. Most people donââ¬â¢t worry about the harm that an unknown Facebook friend, or Twitter follower is capable of; they mostly think ââ¬Å"Yay! A new follower/friend! Iââ¬â¢m so popular!â⬠Well, yeah, maybe theyââ¬â¢re popular in the mind of a predator, but people should still watch out for situations like that because you never know when your life is at risk. Cyber bullying is a form of harassment via social networks; it causes its victims ââ¬â mainly teenagers between the ages of fourteen and seventeen ââ¬â to become emotionally and mentally scarred by its use. The web article ââ¬Å"Are Social Networking Sites Good for Our Society?â⬠updated in September, 2012, urge, social networking sites make cyber bullying ââ¬â a form of bullying that occurs online ââ¬â easier and more public than bullying through other online activities such as email and instant messaging. Cyber bullying can be classified as just commenting on someoneââ¬â¢s picture on Facebook, saying that their hair is really messed up. But other formââ¬â¢s of cyber bullying can be calling someone out in a status, calling them ugly, or fat, or any hurtful word. It can differ, but all forms of it can be hurtful and send anyone of the edge. The web article ââ¬Å"Are Social Networking Sites Good for Our Society?â⬠updated in September, 2012, ââ¬Å"A 2009 study found that 17.3% of middle school students have been victims of cyber bullying; victims often experience a drop in grades, decreased self-esteem, and other symptoms of depression.â⬠Well, kids would drop their self esteem because their self esteem is built by their surrounding peers; and if their peers are nothing but a bunch of bullies then it causes a decrease. The web article ââ¬Å"Cyber Bullying Statisticsâ⬠, published in early 2012, realizes, around half of teens have been the victims of cyber bullying. Many teenagers and children do not care for people who are outside of their groups of friends, so they feel that if they are different than them in any way, or they do things that they donââ¬â¢t do, then they are weird and they donââ¬â¢t like them, so they bully them for being different. Socialnetworks.procon.org, suggests, ââ¬Å"Social networking sites make cyber bullying, a type of bullying that occurs online, easier and more public than bullying through other online activities such as email and instant messagingâ⬠. Itââ¬â¢s easy for a child or a teenager to bully their peers, because kids just donââ¬â¢t care how they make each other feel; they donââ¬â¢t care about anyone but themselves in many situations. Itââ¬â¢s also easy for a kid to call someone a mean name, but even easier to call them a mean name through a computer monitor; probably so they donââ¬â¢t have to deal with a conflict between the victims in person. Cyber predators find their victims through internet sources; which is often social media to learn personal information, harass, and stalk their victims in most cases causing physical, emotional and/or mental harm to them. Michelle Dean, author of the web article ââ¬Å"Amanda Todd: Cyber Bullied,â⬠published in October 2012, comments, ââ¬Å"The systematic way the harasser allegedly followed Todd to new schools, repeatedly posting the images and threatening to do it again, makes it textbook harassment regardless of the medium.â⬠Some Cyber predators will stop at nothing to get what they want from their victim, no matter how far off the edge it will make the victim go; of course predators only think of themselves when it comes to their own wants. The web article ââ¬Å"Killer Created Facebook Profile to Lure Girl to Bushland,â⬠published by Gale Opposing View Points in Context, in 2012, explains, A man [that stalks] a girl on Facebook, learns all about her life and interests to plot how he would murder her. A man kills a woman by making a fake Facebook profile and telling her that he is a part of an animal association, drawing her into his lies by knowing all of her interests because of shared online information. Cyber predators use the internet as their source to figure out any and everything that is in association with their victimââ¬â¢s life. They are a regular predator, searching the internet for, usually young, victims. Victims are younger because they are the ones that donââ¬â¢t really pay any attention to who they do and donââ¬â¢t talk to through the internet, and are the most open to making a conversation with a random person. Communication between others over the internet can be fun and entertaining, but also it can be taken as a way of decline in our communication skills. Jonathan Cummings, author of the article ââ¬Å"___________________â⬠, published by _________ in ____, insists, Communicating through the internet is not as meaningful or reliable as face to face relationships. Although communicating through a computer monitor can be quite engaging, it isnââ¬â¢t as meaningful as chatting with someone in person; you canââ¬â¢t get the fullness of the conversation because you canââ¬â¢t see their emotions and actions. It just isnââ¬â¢t as much fun as a regular, in person conversation. Jonathan Cummings, author of _______, published by ______, in ____, states, ââ¬Å"Using the internet to build social relationships results in social interaction that is wanting, at least when it is explicitly compared to the standards of face-to-face and telephone communication, to social relationships that are primarily conducted offline, and to traditional small groupsâ⬠¦the Internet is less effective than other means of forming and sustaining strong social relationships.â⬠For instance, the internet can give you another form of communicating but it isnââ¬â¢t as insightful as having a conversation with someone in person. Many people are starting to enter the huge world that the internet holds, it may be very fun, but you canââ¬â¢t grasp the meaning of a good conversation when you are lacking one of the senses that most people include in their conversations; usually peopleââ¬â¢s actions or tone is what triggers a good, or meaningful conversation. Social networking has changed how people live, work, and communicate in a positive light by offering helpful schooling online, interesting social communication between others, and negatively by giving bullies and predators another method to victimize others. To help prevent these situations, people could not add others on social media websites that you do not know and are actually friends with. ââ¬Å"Social networking sites have no way to verify that people are who they claim to be, leaving people vulnerable to solicitations from online predators who are able to mask their true identities.â⬠Although it makes anyone seem like theyââ¬â¢re super popular on Facebook when they have around 2,000 friends, they DONââ¬â¢T know them all. Maybe they know about 300 of them personally, but the rest are all people that they donââ¬â¢t even know. Itââ¬â¢s not about having a lot of friends, itââ¬â¢s about having a safe life online. ââ¬Å"Social media can be a powerful tool for social change and an alternative to more traditional methods of communication.â⬠Works Cited Carter, Denise. ââ¬Å"Living in Virtual Communities: Making Friends Onlineâ⬠. Taking Sides: Science, Technology, and Society. Eighth Edition. Thomas Easton. New York, NY. McGraw-Hill. 2008. Print. 15 Sep. 2012. Dean, Michelle. ââ¬Å"Amanda Todd: Cyber Bullied.â⬠The New Yorker. Web. 15 Sep. 2012 Ferguson, Andrew. ââ¬Å"R U Tweeting 2 Much?â⬠Commentary 131.1 (2011): 72+. Gale Opposing Viewpoints In Context. Web. 12 Sep. 2012. ââ¬Å"Killer created fake Facebook profile to lure girl to bushland.â⬠Sydney Morning Herald [Sydney, Australia] 1 Sept. 2012: 5. Gale Opposing Viewpoints In Context. Web. 12 Sep. 2012 ââ¬Å"Social Networking.â⬠SocialNetworking.ProCon.org. ProCon. 12 Aug. 2012. Web. 15 Sep. 2012. Tsukayama, Hayley. ââ¬Å"Dreams of Ray Bradbury: Predictions That Came True.â⬠Washington Post 6 June 2012. Gale Opposing Viewpoints In Context. Web. 12 Sep. 2012.
Monday, January 6, 2020
Essay about Mohandas Ghandi - 1394 Words
Mohandas Ghandi Satyagraha. Meaning force or firmness of truth, Mohandas Gandhi worked and lived by this word. By peaceful, non-violent demonstrations he little by little took hold of the people of Indias love and honor and freed them from British rule. This is his story: On October 2, 1869 in Porbandar, India, a region of Queen Victoria, Mohandas Gandhi was born to Kaba Gandhi and his wife. Although his father, Kaba, was the chief Minister for the Maharaja of Porbandar, he and his family lived in a small house and belonged to a Hindu caste of merchants called banjas. As he grew, Mohandas became a small, shy and skinny boy, afraid of others opinions. He never spoke out, but although he was never a clever child, others wereâ⬠¦show more contentâ⬠¦He had seen the hate the white man had for Indians. And for what reason? Their color or heritage? This did not seem right to him, so he began his life long struggle of never ending peaceful protests and his silent fight for justice for all. Mohandas Gandhi died in January of 1948. (The actual date is in dispute. The information from my book said that his death was on the 30th, but the World Book Encyclopedia says that it was Jan. 13th) He was shot 3 times by Godse, a Hindu himself. The whole world mourned for Gandhi, a man who had no authority in government, but definitely had earned the respect and most importantly, the love of his people. Showing love and humanity through peaceful acts, he became well- known and well-liked. For instance, in 1906 the Zulus in Africa (blacks) rebelled. A new tax had been forced upon them and they had refused to pay and decided to instead, fight. Sadly, they were almost at once crushed by the well-armed white people. There were many wounded so showing as much fortitude as possible, Gandhi and a group of his volunteers marched 40 miles a day through hilly country carrying much needed medical supplies to heal them. At first some of the soldiers wouldnt let him go, but eventually they understood. Some even thanked him. Gandhi also, no matter how they hated this, wanted peace between the Muslims and the Hindus. Their religions were always against eachShow MoreRelated Mohandas Ghandi Essay476 Words à |à 2 Pages Mohandas Ghandi was the source of many changes throughout, India, Britain, and the world. With all that Ghandi has done in our world it becomes overwhelming when I think about his life. What Ghandi did in terms of opening the minds of the people of India is almost analogous to what Christ did to open the minds of the people around him. With all that can be said about Ghandi, I would like to focus upon his economic impact in Britain and India. Britainamp;#8217;s self-glorifying empire buildingRead MoreMachiavelli and Gandhi Essay858 Words à |à 4 PagesAhimsa is a concept that was practiced by political and ideological leader Mohandas Gandhi; a concept that he used to promote the use of non-violent tactics and passive resistance against colonial rule in India. Mohandas Gandhi used Ahimsa as the means to an end and therefore, ultimately rejected Machiavellis advice on the qualities a prince must possess to retain his title. Machiavelli advises that the leaders have military experience and, ââ¬Å"In peace time he must train himself more than in timesRead MoreMahatma Gandhi As A Pragmatism1136 Words à |à 5 PagesMahatma Gandhi (Mohandas Karamchand Ghandi) was born on 2nd October 1869 at Porbander Gujarat. Ghandi was born into a middle-class family, he had his early education at Rajkot, he married to Kasturba. Then he went to England for higher studies. He was an average student in his early childhood. He returned to Ind ia as barrister. He went to south Africa and did practice as a lawyer. He fought for the rights of Indians living there. He left his practice and came back to India. He had worked for theRead MoreMahatma Gandhi : The Battle Of Wandiwash1250 Words à |à 5 PagesMohandas Karamchand Gandhi, also known as Mahatma Gandhi was born on October 2, 1869 in Porbandar, Gujarat. At the age of 19, he studied at Inner Temple law school in London. After graduating, he returned to India and started his own law firm, but found little success. Later on, he joined an Indian firm and was sent to an office in South Africa along with his wife and kids. Discrimination against Indians in South Africa motivated Gandhi to lead an 8-year campaign of civil disobedience. After theRead MoreMahatma Gandhi - Father of Modern India1734 Words à |à 7 PagesMohandas Gandhi ââ¬â Father of Modern India ââ¬â Sky W. During the late 1800ââ¬â¢s, India was yet again being taken over by another conquering nation (Britain). The British were not the first to do this, but followed in the footsteps of the Greek and Persian invasions of the 5th Century BC. Though the control factor remained the same, the way the British went about doing it ââ¬â gradual and subtle ââ¬â was not the same method the Persians or the Greeks used of an immediate and simple takeover. If India were toRead MoreViolence And Violence Is Around Us Throughout The World Essay2034 Words à |à 9 Pagesa positive mechanism to human survival. However, when we think of religion and violence we do not always situate them with each other. This is mainly because we are taught that all religions have an agreed goal for peace. The Indian pacifist Mohandas Ghandi once said, ââ¬Å"An eye for an eye only ends up making the whole world blindâ⬠(Gandhi). He came on this earth with his message of truth and non-violence; Truth and no n-violence are the most fundamental aspect of his thoughts. Carter claimed, ââ¬Å"He hadRead More Gandhi Was One of the Greatest Men to Ever Live Essay1163 Words à |à 5 Pagesworld. A man, who people can surely call the greatest, should be a universal man ââ¬â a man who combines in perfect balance the supreme qualities of an idealist and a realist, a dreamer and a doer. The man who satisfies those qualities, I believe is Mohandas Karamchand Gandhi, the Indian leader, of the great revolutionary movement against British rule in India. He is better known as Mahatma, called by his own countrymen first, meaning ââ¬Å"the Saintâ⬠. Gandhi was born on second October, 1869 in India, ofRead MoreGandhi : The World Of Mahatma Gandhi1320 Words à |à 6 PagesEvan Zuniga Professor Krupnik Comparative World Religions May 6, 2016 Research Paper: Gandhi Mohandas Karamchand Ghandi, better known to the world as Mahatma Gandhi is one of the worldââ¬â¢s main faces when we think or talk of the Indian independence movements, womenââ¬â¢s rights and all around freedom for humanity. This individual used strategies and tactics of his own to achieve justice for the Indian culture while he was alive. Gandhi also worked to reform traditional Indian society inRead MoreEssay On Mahatma Gandhi1859 Words à |à 8 PagesOctober 2, 1869 in the small western Indian state of Porbandar under the name Mohandas Karamchand Gandhi (Ghandi s Life) . When Gandhi was finished with high school, he entered a small Indian college, the Samaldas College at the University of Bombay (Mahatma). After beginning his education here, however, he decided he disliked and traveled to the University College London, leaving his wife and infant son (Ghandi s Life). It was while attending school in London that he initially became exposedRead MoreGandhis acts of civil diso bedience Essay2654 Words à |à 11 PagesMohandas Karamchand Gandhi In 1757, Great Britain extended its empire into India. This occupation would not fully end until 1947. In the time between, there were many movements by the Indian people to gain independence from the British. The movement that finally succeeded in winning Indiaââ¬â¢s independence was led by one of the most influential figures of the 20th century, Mohandas Karamchand Gandhi. Gandhiââ¬â¢s methods for fighting against the occupation of the British were very different from
Sunday, December 29, 2019
Phosphate-Buffered Saline or PBS Solution
PBS or phosphate-buffered saline is a buffer solution that is particularly valuable because it mimic the ion concentration, osmolarity, and pH of human body fluids. In other words, its isotonic to human solutions, so its less likely to cause cell damage, toxicity,à or unwanted precipitation in biological, medical, or biochemical research. PBS Chemical Composition There are several recipes to prepare PBS solution. The essential solution contains water, sodium hydrogen phosphate, and sodium chloride. Some preparations contain potassium chloride and potassium dihydrogen phosphate. EDTA may also be added in cellular preparation to prevent clumping. Phosphate-buffered saline is not ideal for use in solutions that contain divalent cations (Fe2, Zn2) because precipitation mayà occur. However, some PBS solutions do contain calcium or magnesium. Also, keep in mind phosphate may inhibit enzymatic reactions. Be particularly aware of this potential disadvantage when working with DNA. While PBS is excellent for physiological science, be aware the phosphate in a PBS-buffered sample may precipitate if the sample is mixed with ethanol. A typical chemical composition of 1X PBS has a final concentration ofà 10 mM PO43âËâ, 137 mM NaCl, and 2.7 mM KCl. Heres the final concentration of reagents in the solution: Salt Concentration (mmol/L) Concentration (g/L) NaCl 137 8.0 KCl 2.7 0.2 Na2HPO4 10 1.42 KH2PO4 1.8 0.24 Protocolà for Making Phosphate-Buffered Saline Depending on your purpose, you may prepare 1X, 5X, or 10X PBS. Many people simply purchase PBS buffer tablets, dissolve them in distilled water, and adjust the pH as needed with hydrochloric acid or sodium hydroxide. However, its easy to make the solution from scratch. Here are recipes for 1X and 10X phosphate-buffered saline: Reagent Amountto add (1Ãâ") Final concentration (1Ãâ") Amount to add (10Ãâ") Final concentration (10Ãâ") NaCl 8 g 137 mM 80 g 1.37 M KCl 0.2 g 2.7 mM 2 g 27 mM Na2HPO4 1.44 g 10 mM 14.4 g 100 mM KH2PO4 0.24 g 1.8 mM 2.4 g 18 mM Optional: CaCl2â⬠¢2H2O 0.133 g 1 mM 1.33 g 10 mM MgCl2â⬠¢6H2O 0.10 g 0.5 mM 1.0 g 5 mM Dissolve the reagent salts in 800 ml distilled water.Adjust the pH to the desired level with hydrochloric acid. Usually this is 7.4 or 7.2. Use a pH meter to measure the pH, not pH paper or other imprecise technique.Add distilled water to achieve a final volume of 1 liter. Sterilization and Storage of PBS Solution Sterilization isnt necessary for some applications, but if your are sterilizing it, dispense the solution into aliquots and autoclave for 20 minutes at 15 psi (1.05 kg/cm2) or use filter sterilization. Phosphate-buffered saline may be stored at room temperature. It may also be refrigerated, but 5X and 10X solution may precipitate when cooled. If you must chill a concentrated solution, first store it at room temperature until you are certain the salts have completely dissolved. If precipitation does occur, warming the temperature will bring them back into solution. Shelf life of refrigerated solution is 1 month. Diluting a 10X Solution to Make 1X PBS 10X is a concentrated or stock solution, which may be diluted to make a 1X or normal solution. A 5X solution must be diluted 5 times to make a normal dilution, while a 10X solution must be diluted 10 times. To prepare a 1 liter working solution of 1X PBS from a 10X PBS solution, add 100 ml of the 10X solution to 900 ml of water. This only changes the concentration of the solution, not the gram or molar amount of the reagents. The pH should be unaffected.à PBS Versus DPBS Another popular buffer solution is Dulbeccos phosphate buffered saline or DPBS. DPBS, like PBS, is used for biological research and buffers in the 7.2 to 7.6 pH range. It can be stored at room temperature. Dulbeccos solution contains a lower concentration of phosphate. It is 8.1 mM mM phosphate ions, while regular PBS is 10 mM phosphate.à The recipe for 1x DPBS is: Reagent Amount to add (1x) NaCl 8.007 g KCl 0.201 g Na2HPO4 1.150 g KH2PO4 0.200 g Optional: CaCl2â⬠¢2H2O 0.133 g MgCl2â⬠¢6H2O 0.102 g Dissolve the salts in 800 mL of water. Adjust pH to 7.2 to 76 using hydrochloric acid. Adjust the final volume to 1000 mL with water. Autoclave at 121à °C for 20 minutes. Sources Dulbecco, R.; et al. (1954). Plaque formation and isolation of pure lines with poliomyelitis viruses. J. Exp. Med. 99 (2): 167ââ¬â182.Phosphate-buffered saline (PBS. Cold Spring Harbor Protocols (2006). Cold Spring Harbor Laboratory Press.
Saturday, December 21, 2019
People Will Do Anything For Power - 1303 Words
People will do anything for power. All over the world there are many different types of power. Some countries have presidents, some have kings and queens, and some have gods. Julius Caesar was a man that loves power. He loved thinking of himself above everyone else, and everyone else adored him. The towns people cheered for him and decorated the streets with his name. What Caesar didnââ¬â¢t know was that there are some people that didnââ¬â¢t want him to come to power, and some of them might just be the Roman Tribunes. A Tribune was a Roman official whose job was to protect the people against oppression by the patricians or nobles. The word tribune is connected to the word tribe. In Latin the word for Tribune was tribunus, which means an officer connected with a tribe, or someone that represented a tribe for specific purposes. Originally, a tribune represented a tribe of rulers then later it became known as a variety of officers. The tribunes were at first one, but their number was increased ultimately to ten. The two most important were the tribunes of plebs and the military tribunes. A Roman Tribune was a very high position. In certain ranks you had certain jobs, for example if my ranking was Tribune my job was to assist the legion commander, the Primus Pilus. If my ranking was Tribunus Cohortis I was the commander of a Cohort military unit. Being a Roman Tribune gave you the right to veto laws, command portions of the army, and eventually callShow MoreRelatedSpanish Dbq Essay649 Words à |à 3 Pagesonly focusing on gold, glory, and God, but by strength and power as well. The age of exploration to the new world was dominated by the Spanish conquistadors. Their success in new discoveries brought great power and strength to Spain. In most circumstances, a country would do anything to become successful in the economy and government. With that being said, Spain every intention based on becoming powerful, which left no thought toward the people living in the New World. God and religion was such anRead MoreThe Crucible By Arthur Miller1273 Words à |à 6 Pagesgeneral members of society will do anything to attain or maintain a position of power and status amongst the bourgeoisie while a few will go through great lengths to remain true to their faith; ultimately revealing that the act of trying to achieve a higher position in life, at the cost of others, is a choice. Throughout the story you see characters change their views, opinions, and actions on certain items just to remain in the position that they have achieved. They also do these things to get to a higherRead More Utopia by Thomas More and The Prince by Machiavelli Essay821 Words à |à 4 Pageslies in the way the authors handle the subject of power. As a manual, or handbook if you will, Prince treats power as a necessity, a goal, to be worked towards and maintained, almost at all costs. Utopia, a fantasy, treats power as something all individuals have; rather, they are empowered. By comparing the way both works use and treat point of view and form, governmental systems and ideals the differences in perspective on power becomes clearer. Ideas are brought forth differentlyRead MoreFahrenheit 451 By Ray Bradbury And The Catching Fire By Suzanne Collins1464 Words à |à 6 Pagesgovernment and leaves no power to the people. Two examples of dystopias would be take place in Fahrenheit 451 by Ray Bradbury and in Catching Fire by Suzanne Collins. They have an abundance of components in common, while there are also a few notable differences, each society has its own advantage, and the reactions would be different depending on what dystopia and the surroundings you are placed in. They both portray possible future societies. In both societies, the people are constantly watchedRead MoreFrankenstein Fate vs Free Will857 Words à |à 4 Pagesï » ¿Frankenstein Oh how has Hollywood changed the story and lost the meanings of Frankenstein, for the themes have been missed by many people that have only seen the movies and not have read the book. One such theme Mary Shelly gives the reader is the power of Fate versus Free Will. Victor is found by Robert Walton in the artic while Victor is trying to capture a monster that he has created. Victor flashes back to his past and tells Robert how he created the monster and how the monster killedRead MoreImportance Of Education Is Education1277 Words à |à 6 PagesPower, Privilege, And Responsibility is Education Imagine what the world will be like without education. Malcolm X states that ââ¬Å"without education, you are not going anywhere in this world.â⬠People think they will get through life without education, but little do they know the importance of it. Educated individuals have power because they are able to understand complex info and are more informed to prevent being taken advantage of. It is also a privilege because not everyone has access to educationRead More Absolute Power Does NOT Corrupt Absolutely Essay840 Words à |à 4 PagesA man named Lord Actor once said, Power tends to corrupt, and absolute power corrupts absolutely. Great men are almost always bad men. This means that giving a person some power can turn them into a bad person. However, giving someone absolute power will always corrupt some and that these people are always bad. Knowing what the quote means the question remains, is the quote true? The answer is no. Power tends to corrupt, and absolute power corrup ts absolutely. Great men are almost always bad menRead MoreAldous Huxley s Brave New World875 Words à |à 4 Pagesbeginning of time, humans have strived to be happy. During this time, thousands of different people have given their interpretation of happiness. According to the Merriam-Webster dictionary, the simplified definition of happiness is feeling pleasure or joy because of a certain situation. In Aldous Huxleyââ¬â¢s Brave New World, Controller, Mustapha Mond, conceals the truth in order to keep everyone happy. He gives his people drugs and brainwashes them into believing that their life is good. Satisfying. When JohnRead MoreA Successful Communist Utopia Is A Dream Because It Would Require All Non Communists1181 Words à |à 5 Pagesquestion is often, how do these rulers gain so much power? Stalinââ¬â¢s rise to power began in 1905 when he attended a conference in Finland as a delegate. Joseph Stalin is then appointed to the Partyââ¬â¢s Central Committee by Vladimir Lenin in 1912, and in 1922, he was chosen as General Secretary of the Communist Party. Stalin took the path of slowly working his way up the hierarchy to gain his own power (Joseph Stalin Timeline). Big Brother was the figure of power in 1984, he gained said power because the governmentRead MoreFear Of Jews In The Storyteller By Jodi Picoult724 Words à |à 3 Pagestowards making terrible decisions are power and fear. Hitler continued to kill as many other non-Germans (especially Jews) because he wanted to gain power over as many people as he could. The reason that the majority of the Nazi soldiers went through with these killings was because they were terrified of being executed by their leaders. Even though many people commit awful crimes, not all of them are bad people because of the pressure that was placed on them to do it. Most of the injustices that occur
Thursday, December 12, 2019
Commercial and Corporation Law Trading Parties
Question: Discuss about theCommercial and Corporation Lawfor Trading Parties. Answer: Introduction Commercial law refers to set of laws which stipulate the rights and guide relations and conduct of people and businesses engaged in trade and other business operations that are engaged in profit generating activities. Commercial law determines the rights and responsibilities of the trading parties, governs resolution of disputes arising from trade among other factors arising from business operations (Fu, 2007). On the other hand corporate law is the study of the relationship and interaction of various parties involved in business including customers, stakeholders, shareholders, the environment, company directors, and creditors among others ( Keay, 2011). It is basically the law that governs operations. PART A An entrenchment provision is a clause that allows or prohibits certain amendments of basic laws or the constitution for the purposes of protecting the rights of parties especially the minority. In business this is particularly important to maintain good relationship between the trading parties. David may want to have such provisions included in the companys constitution for a number of reasons. Any company operating in an area must be socially responsible to both its internal and external environments. This means that its operations must be in line with the expectations of both the external and internal environments as stipulated in the companys constitution or other laws guiding business operations. David could therefore be aiming at ensuring that business operations are mindful of both their internal and external environments. In business there are always the majority and minority groups. Whenever there are such associations there is always the tendency of the majority group to dominate over the minority of group hence infringing on their rights. In Organizations, the minority might be either employees or the public because in most cases they have to live by the decisions that are made by the management. Entrenchment provisions are therefore important to prevent the management from making dictatorial decisions that can negatively affect the minority groups. David could have therefore wanted the provisions to be put in place to ensure that these groups enjoyed their full rights. The provision also promotes stability. Whenever there are unregulated business operations disputes are likely to occur. In situation where the minority groups feel oppressed through unethical business operations such as unfair wages, unfair prices for goods and services among others they are likely to result into protests and pickets which hinders normal business operations. Such protests are likely to cause instability. David could have been aiming reducing such protest which in effect would lead to stability in business operations. PART B In any business operations each person has their duties and responsibilities clearly stipulated in their job description. Basically the job description is what guides the operations of each employee (Fazio, 2007). Basically a receptionist is mostly authorized to welcome visitors, answering phone calls, appointment calendars, receiving and sorting deliveries and performing of clerical duties among others. For any purchase to be termed as legal it must be budgeted for and approved by the financial officer. Any other route followed in making orders for purchases is not allowed. The receptionist has no authority for making any orders for goods and services on behalf of the company. This is basically the role of the purchase management department. By ordering for such goods the receptionist will be indulging in responsibilities that are not within his or mandate. In my opinion I think that the company is not legally bound to pay for the goods. This opinion is based on a number of factors .First of all, all purchase orders within an organization must meet the stipulated criteria for requesting for goods and services. Basically all purchases within an organization are made by the purchases department or in other departments the role is given to the procurement department .This means that all purchase requests must be forwarded to these departments for approval and implementation. All departments are required to establish all goods and services that are needed within department and prepare a budget for the goods then fill a purchase request form which is then forwarded to the procurement and finance departments for approval. The purchases department establishes the necessity of the goods or a service requested and later approves or rejects the request based on the necessity. If the request is approved, it is then forwarded to the finance department so that the budget can be approved and for payment to be authorized. The finance department can decline or accept the request depending on their ability to pay for the goods and services. This is the approved procedure for requesting for goods and services In Michelles case however, this procedure was not followed, so the company was not bound to pay office works. References: Keay, a. (2011). The corporate objective. Cheltenham, edward elgar pub. Http://public.eblib.com/choice/publicfullrecord.aspx?p=714169. Hanrahan, p. F., ramsay, i. M., stapledon, g. P. (2016). Commercial applications of company law. Fu, j. (2007). Commercial and corporations law. Geelong, vic, deakin university. Fazio, s. (2007). The harmonization of international commercial law. Alphen aan den rijn, the netherlands, kluwer law international. Tomasic, r., bottomley, s., mcqueen, r. (2002). Corporations law in australia. Sydney, federation press. Ma?ntysaari, p. (2012). Organising the firm: theories of commercial law, corporate governance and corporate law. Dordrecht, springer. Http://public.eblib.com/choice/publicfullrecord.aspx?p=885119.
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